瘦素受体基因Gln 223 Arg变异与武汉地区肥胖合并高血压患者相关性研究

Association of Gln 223 Arg variant in leptin receptor gene in Obesity with hypertension in Wuhan “Han” population

目的研究瘦素受体基因Gln 223 Arg变异与肥胖合并高血压的关系。方法运用聚合酶链反应-限制性片段长度多态性(PCR-RELP)方法测定无亲缘关系,且有完整临床资料的766例武汉地区汉人的瘦素受体基因Gln 223 Arg变异的基因型(包括252例正常对照者及肥胖合并高血压256例,非肥胖高血压患者136例,单纯肥胖者122例)。结果肥胖合并高血压不同性别,不同瘦素受体基因Gln 223 Arg变异的高血压合并频率比较发现,瘦素受体基因Gln 223Arg变异与男性肥胖合并高血压相关,A等位基因与男性较高的收缩压(P=0.023)及舒张压(P=0.036)及BMI(P=0.031)相关。Logistic回归分析证实,该基因变异是肥胖男性合并高血压的独立风险因子(P=0.036)。携带“A”等位基因的男性肥胖合并高血压发生的比数比(OR)为2.711(95%可信限为1.216、5.129)。结论瘦素受体基因Gln 223 Arg变异与肥胖男性合并高血压相关,收缩压和舒张压及BMI均相关。

Objeetive To investigate the association of Gin 223 Arg variant in leptin receptor gene in obese patients with hypertension. Methods The genotypes of Gin223Arg variant （PCR-RFLP） assay in 766 unrelated subjects of WUHan ＂Han＂ population （ including 252subjects control and 256 subjeets obese with hypertension patients and 136 subjects not obese hypertension patients,and122 subjects obese without hypertension patients）. Results Signifieant difference of genotype frequency of Gln223 Arg variant in lepfin receptor gene was observed between obese with hypertension and male patients , ＂ A＂ allele was associated with increased systolic blood pressure （ P = 0. 023 ） and diastolic blood pressure （ P = 0.036） and BMI （P = 0. 031 ） in obese with hypertension male patients. Logistic regression analysis showed that this gene variant was an independent risk factor of obese with hypertension patients male patients accompanied, with hypertension The odds ratio of hypertension in obese with hypertension patients with ＂A＂ allele was 2. 711 （95% CI 1. 216.5. 129）. Conclusion Gln223Arg variant in leptin receptor gene is associated with hypertension in obese with hypertension patients male patients, espeeiaUy with elevation of systolic blood pressure and diastolic blood pressure and BMI.