低浓度戊二醛对无菌性脑膜炎暴发病原Echo30毒株的杀灭效果评价

Evaluation of virucidal efficacy of glutaraldehyde at low concentrations against pathogenetic virus Echo30 of an aseptic meningitis outbreak

目的:评价低浓度(<2%)戊二醛消毒剂对2003年苏北儿童无菌性脑膜炎暴发病原Echo30-FD JS03-102分离株病毒的灭活效果。方法:用悬液法测定Echo30-FD JS03-102接触0.05%,0.1%,0.2%戊二醛前后病毒滴度对数值的改变情况。观察不同pH环境下病毒灭活率的差别。结果:25℃pH 7.2条件下,戊二醛对Echo30-FD JS03-102的灭活速度随浓度增加而升高。pH8.3的弱碱性环境能够提高低浓度戊二醛的消毒效果。结论:Echo30-FD JS03-102对低浓度戊二醛具有一定的抵抗力;但在合适的条件下戊二醛仍能达到满意的消毒效果。

Objective： To assess virucidal efficacy of ghtaraldehyde （GTA） at low concentrations against isolate strain Echo30 - FDJS03 - 102, one of which were pathogen of an aseptic meningitis outbreak in north area of China, 2003. Methods：Suspension procedure was used to measure changes in logl0 value of TCID50 of virus after mixed with GTA at different concentrations of 0. 05%, 0. 1%, 0.2%. Influence of pH on inactivation rate of virus was also watched. Results：Inactivation speed was enhancing with concentration increase of GTA at pH 7.2,25℃. At pH 8. 3, virucidal efficacy could be boosted compared with which at pH 7.2. Conclusion：Echo30 -FDJS03 -102 can partially resist against GTA at low concentrations. Satisfying efficacy of GTA can be achieved once essential conditions are met.