摘要
目的:探讨选择性COX-2抑制剂Nimesulide对人胰腺癌PANC-1细胞增殖和凋亡的影响及其可能分子机制。方法:应用四甲基偶氮唑蓝(MTT)法观察不同浓度Nimesulide对PANC-1细胞增殖的影响;流式细胞术(FACS)及DNA梯状电泳(DNA ladder)检测PANC-1细胞凋亡的改变;Western blotting法检测Nimesulide不同浓度作用下PANC-1细胞COX-2蛋白水平的改变,RT-PCR法检测COX-2 mRNA水平的变化。结果:MTT、流式细胞术及DNA ladder显示Nimesulide呈剂量依赖性地抑制PANC-1细胞的增殖,并诱导其凋亡;Western blotting和RT-PCR法显示随着药物浓度的增加,COX-2蛋白及mRNA表达降低。结论:Nimesulide可能通过对COX-2表达的下调而诱导凋亡,从而抑制人胰腺癌PANC-1细胞生长。
Objective: To study the effects of selective eyclooxygenase - 2 ( COX - 2) inhibitor, nimesulide on the proliferation and apoptosis of human pancreatic carcinoma PANC - 1 cell line and its possible molecular mechanisms. Methods: The effect of nimesulide on cell proliferation was assessed by MTT assay. Apoptosis was determined by FACS analysis and DNA ladder. Western blotting and reverse transcriptase- polymerase chain reaction (RT- PCR) were used to demonstrate expression level of COX -2 protein and mRNA. Results:MTT, FACS and DNA ladder showed that nimesulide significantly decreased cell viability and induced apoptosis in pancreatic tumor PANC - 1 cell line. Western blotting and RT - PCR revealed that the protein and mRNA expression of COX - 2 were reduced by high concentration of nimesulide. Conclusion: COX - 2 selective inhibitor nimesulide inhibits proliferation, induces apoptosis of pancreatic carcinoma PANC -1 ceils via down- regulation of COX-2 expresstion.
出处
《中国卫生检验杂志》
CAS
2007年第5期810-812,共3页
Chinese Journal of Health Laboratory Technology