HIV抗体干血浆滤纸片质控品制备的研究

Study on HIV antibody detection control-specimens by filter paper with dried plasma spot

目的:研究制备一种新型的干血浆滤纸片质控品,用于艾滋病初筛实验室间质量评价活动。方法:取来自中国地区不同亚型的HIV抗体强阳性标本,分别制成5份标本。将这5份标本分别进行HIV抗体效价滴定。最后选取OD值高低不同的5份标本作为室间质量评价的标本。结果:用ELISA法检测HIV抗体,干血浆滤纸片样品与血浆样品检测结果无显著性差异(P>0.05)。结论:干血浆滤纸片质控品可以用于基于ELISA方法检测的HIV抗体的初筛实验室的质量评价活动中,其在稳定性、样品保存、运输方面均优于血浆质控品。

Objective ：To probe the feasibihty of the human immunodeficiency vires （HIV） antibody detection through dried plasma spots control - specimens on filter paper instead of plasma specimens. Methods： Mix well many plasma from HIV infectors, which was processed into dried plasma spot control - specimens on filter paper and the plasma control - specimens. Results： The specimens of dried plasmas spot （DPS） on filter paper and the plasma control - specimens were tested about antibodies of the human immunedeficiency vires with ELISA assay. There was no difference between two groups （ P 〉 0. 05 ）. Conclusion： DPS is superior in stability, specimen preservation and transportation and it may be used to evaluate HIV antibody detected by ELISA between different screening laboratories.