丙溴磷农药对油菜抗氧化酶活性的影响

Effects of profenofos stress on activity of POD and CAT of brassica napus

目的:研究在丙溴磷农药作用下,油菜的抗氧化酶系统(过氧化物酶POD、过氧化氢酶CAT)的活性变化。方法:POD与CAT的活性分别采用愈创木酚法和紫外吸收法。结果:丙溴磷对油菜的POD、CAT活性影响显著,处理组POD、CAT活性明显高于对照组,CAT活性在实验天数内呈现先升高后下降的趋势。结论:在油菜遭受丙溴磷胁迫初期POD和CAT活性即变化显著,可作为丙溴磷迫胁油菜比较敏感的指标。

Objective：An experiment was carried out to study the effects of profenofos stress on the activities of catalase （CAT） and peroxidase （POD） in Brassica Napus. Methods： The activities of POD and CAT were detected by speetrophotometry. Results：The activities of CAT and POD were all promoted under the profenofos stress. Prolonged duration of profenofos stress increased the activities of CAT, which soon declined. Conclusion：The activities of CAT and POD which were all highly improved could serve as sensitive biomarker under profenofos stress in Brassica Napus.