摘要
实验通过DEAE离子交换层析对牛背长肌中钙激活酶(Calpains)的分离研究,比较了DEAE-Sepharose-FF和DEAE-Sephacel对钙激活酶(Calpains)的分离效果,确定了Calpains分离纯化的条件。Sephacel不耐压,分离时间长,因此选择Sepharose-FF分离纯化Calpains。
The objective of this experiment was to study Calpains activities of bovine M. Iongissimus and find conditions of purification of Calpain system by ionexchange chromatography. DEAE-Sephacel and DEAE-Sepharose-FF were compared to quantify Calpains activities for comparative purpose. The resμLts indicated that DEAE-Sepharose-FF was superior to DEAE-Sephacel.
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第5期176-177,244,共3页
Science and Technology of Food Industry
关键词
钙激活酶
离子交换层析
分离纯化
Calpain activities
ion-exchange chromatography
abruption and purification
