以盐酸去甲万古霉素为手性选择剂毛细管电泳法分离西替利嗪对映体

Chiral resolution of cetirizine by capillary electrochromatography using norvancomycin hydrochloride as chiral selector

目的:建立以阳离子表面活性剂碘化四丁基铵为电渗流改性剂,以盐酸去甲万古霉素为手性选择剂的毛细管电泳法分离西替利嗪对映体。方法:考察了盐酸去甲万古霉素浓度、Tris 浓度和缓冲液 pH 对分离的影响,对分离条件进行了优化。结果:在含0.04 g·L^(-1)碘化四丁基铵和1.0 mmol·L^(-1)盐酸去甲万古霉素的25 mmol·L^(-1)Tris 磷酸缓冲液(pH 4.5)的运行电解质体系中,西替利嗪对映体在分离电压为20 kV 的条件下得到良好分离,西替利嗪对映体分离度达1.8。结论:本法可用于西替利嗪对映体的分离。

Objective：To establish a capillary electrochromatography method for separation of cetirizine enantiomers, and using cationic surfactant and chiral selector of norvancomycin hydrochloride as running buffer. Methods： The effects of pH and the concentrations of norvancomycin hydrochloride and Tris on chiral resolution were exam- ined and separation conditions were optimized. Results：It was observed that a good separation of enantiomers for cetirizine could be achieved in 25 mmol·L^-1 Tris - H3PO4 buffer system（pH 4.5） containing 1.0 mmol·L^-1 norvancomycin and 0.04 g·L^-1 tetrabutyl ammonium iodide. The resolution between R - cetirizine and S - cetirizine was 1.8. Conclusion ：The method can separate enantiomers for for cetirizine.