不同地区商品沙苑子中沙苑子苷A的含量比较

Compare with the content of complanatoside A of commercial Semen Astragali Complanati in different districts

目的:以沙苑子苷 A 为指标,对不同地区商品沙苑子进行含量测定及质量评价。方法:运用高效液相色谱法,采用 Hy-persil ODS 2(5 μm,4.6 mm×250 mm)色谱柱,以乙腈-水-磷酸(20:80:0.2)为流动相,流速1.0 mL·min^(-1),检测波长为267 nm,测定不同地区市售沙苑子样品中沙苑子苷 A 的含量。结果:各地市售沙苑子的植物来源不同,沙苑子苷 A 的含量差异较大。结论:正品中以陕西渭南所售沙苑子中沙苑子苷 A 含量最高,与我国古代确认的道地药材相吻合;不同地区沙苑子中沙苑子苷 A 含量可作为药材质量评价的一项指标,该方法灵敏、准确。

Objective：Use the method of HPLC to determine the content of complanatoside A in commercial Semen Astragali Complanati in different districts. Method：An HPLC method has been developed to determine complanatoside A in Semen Astragali Complanati from different districts. The mobile phase was acetonitrile -water- phosphoric acid （20：80 ： 0. 2）at a flow rate of 1.0 mL·min^-1 ;the detected wavelength was 267 nm. Result：The complanatoside A content of Semen Astragali Complanati was markedly different compared with different districts. Conclusion： The Semen Astragali Complanati in Shaanxi has the highest content of complanatoside A and the method is stability ,fast and accurate.