HPLC测定风湿安泰片中马钱子碱和士的宁的含量

RP-HPLC determination of strychnine and brucine in Fengshi Antai tablets

目的:采用反相高效液相色谱法测定风湿安泰片中士的宁和马钱子碱的含量。方法:采用 Hypersil BDS C_(18)色谱柱(4.6 mm×250 mm,5 μm),保护柱(4.6 mm×12 mm);乙腈-0.3%三乙胺溶液(磷酸调 pH=2.6)(10:90)为流动相,流速:1.0 mL·min^(-1),柱温:室温,检测波长:254 nm。结果:士的宁在4.6～46μg·mL^(-1)(r=0.9998),马钱子碱在2.65～26.5μg·mL^(-1)(r=0.9998)范围内呈良好的线性关系,士的宁的平均回收率(n=5)为101.1%,RSD 为2.8%;马钱子碱的平均回收率(n=5)为99.6%,RSD 为2.9%。结论:本方法准确、简便、快速,适用于风湿安泰片的质量控制。

Objective：To develop an RP - HPLC method for determination of strychnine and brucine in Fengshi Antai tablets. Method：A Hypersil BDS C18（250 mm×4. 6 mm,5 μn） column was used,and the mobile phase consisted of acetonitrile-0. 3% triethylamine in water（ adjusted to pH =2. 6 with phosphoric acid ） （10： 90）. The flow rate was 1.0 mL·min^-1 ,and the detection wavelength was 254 nm. Results：The calibration curve was linear （ r = 0. 9998 ） within the range of 4. 6 - 46 μg·mL^-1 for strychnine, the average recovery （ n = 5 ） was 101.1% and RSD was 2. 8%. The calibration curve was linear（r =0. 9998） within the range of 2. 65 -26.5 μg·mL^-1 for brucine, the average recovery （ n = 5 ） was 99.6% and RSD was 2. 9 %. Conclusion： This method is accurate, simple, quick and the assay result is suitable for the quality control for strychnine and brucine in Fengshi Antai tablets.