摘要
分别采用分光光度法、菌体量法及孔扩散法研究了乳过氧化物酶(简写LP)对细菌和霉菌的抑菌活性及LP浓度、pH值和温度对抑菌活性的影响。结果表明:LP对于各供试菌均有很好的抑菌效果。其中,LP对铜绿假单胞菌、金黄色葡萄球菌和产黄青霉的抑制作用最显著,5μg/mL就能完全抑制他们的生长;在中性偏酸性环境中的抑菌活性比碱性环境的抑菌活性强;一定温度内处理不影响LP的抑菌活性;在中性条件下抑制供试革兰氏阴性菌和霉菌的能力强于Nisin。
The anti-microbial activities of LP temperature were studied by spectrophotometric and the effect factors including concentration, pH, investigation, aperture pervasion method, and dry cell weight investigation. The results showed that LP had strong inhibition on bacteria and mold activities. Especially on Pseudomonasaeruginosa, Staphylococcus aureus and Penicillium chrysogenum, the complete anti-microbial concentration was 5μg/mL. Anti-microbial activities in neutral and low acid environment were stronger than those in the high alkali environment. Anti-microbial activities of LP would not be affected in a certain temperature scope. Anti-G- and anti-mold activities of LP are better than Nisin in neutral environment.
出处
《乳业科学与技术》
2007年第3期115-118,共4页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
