耐药性癫癎患者颞叶中细胞周期依赖性蛋白激酶5的表达

Expression of cyclin dependent kinases-5 in the temporal lobes of the drug resistance epilepsy patients

目的研究细胞周期依赖性蛋白激酶5(cyclin dependent kinases 5,CDK5)在耐药性癫癎患者颞叶中的表达,探索其在耐药性癫癎发病机制中的作用。方法收集耐药性癫癎患者术后脑组织,用荧光定量 PCR、免疫组化和 Western blot 3种检测方法从基因和蛋白水平分别测定 CDK5在耐药性癫癎患者颞叶中的表达,并与对照组进行比较。结果荧光定量 PCR 发现 CDK5 mRAN 比对照组明显增加,免疫组化检测显示这种基因的蛋白表达产物主要分布在神经元轴突和胶质细胞中,Western blot 检测在相对分子质量35 000处有一蛋白条带,并且可见实验组(颞叶和海马中分别为1.4293±0.1839和2.0733±0.4738)高于对照组(颞叶和海马中分别为0.9680±0.4147和1.403±0.6163,P<0.05)。结论 CDK5在耐药性癫癎患者颞叶中表达增强,提示他们可能参与了耐药性癫癎的形成。

Objective To investigate the expression of cyclin dependent kinases 5 （CDK5） in the temporal lobes of the epilepsy patients and to explore the possible roles of CDK5 in the pathogenesis of refractory epilepsy. Methods The brain tissues of intractable epilepsy （IE） were studied by fluorescence quantative polymerase chain reaction（FQ-PCR） for CDK5 mRNA, while immunohistochemistry and Western blot were used to study the protein expression. Nonepileptogenic control brain tissues were used for comparison. Results FQ-PCR analysis showed that the expression of CDK5 mRNA in epilepsy patients was significant higher than those in the control group. And immunohistochemistry showed that the protein mainly existed in the neuron and glial. At the 35 000 relative molecular mass, Western blot could been seen that there is a limpid strap. The optical density of CDK5 in IE （temporal lobe 1. 4293 ±0. 1839, hippocampus 2. 0733 ± 0. 4738 ） was significantly higher than that in the control （ temporal lobe 0. 9680 ± 0. 4147, hippocampus 1. 4030 ±0. 6160, P 〈0. 05）. Conclusions Upregulation of CDK5 was seen in the patients with epilepsy. A pathogenetic role of CDK5 in epilepsy patients might be included.