乙型肝炎患者及其配偶、子女HBV血清标志物检测结果分析

Detection of the HBV Serum Markers among the Hepatitis B Patients and Their Family Members

目的分析乙型肝炎(乙肝)患者及其配偶、子女血清中的HBV-DNA和血清标志物的检测结果。方法ELISA法和荧光PCR法进行HBVM(乙肝血清标志物5项)与HBV-DNA的测定。结果HBV-DNA的阳性率和含量在HBcAg(+)与HBeAg(-)组差异有统计学意义(χ2=18.21,P<0.01;χ2=156.11,P<0.01)。乙肝患者配偶的HBV感染率为50.56%,母婴与父子女之间的传播差异有统计学意义(χ2=357.97,P<0.01)。结论HBeAg(+)与HBV-DNA的含量有密切关系,母婴传播远高于父子女传播,家庭传播是导致乙肝、肝硬化、肝坏死和肝癌的主要原因之一,而配偶之间的密切接触可感染HBV,但很少引起乙肝。

[ Objective ] To analyze the detection result of the HBV serum markers among the hepatitis B patients and their family members. [ Methods] ELISA and fluorescence PCR were adopted to determine HBVM and HBV -DNA. [ Results] Significant difference was found in the HBV - DNA positive rate and content between the HBeAg（＋） group and HBeAg（-） group （ P 〈0. 01 ） ; the HBV positive rate among the patients＇ spouses was 50.56% ; the difference was significant between mother - infant spread and father - infant spread （ P 〈 0.01 ）. [ Conclusion ] HBeAg （ ＋ ） is closely related with the content of HBV - DNA; mother - infant spread is much more serious than father - infant spread ; spread among family members is one of the major causes of hepatitis B, hepatic necrosis, cirrhosis and hepatic cancer; close contact between spouses might lead to HBV infection but seldom causes hepatitis B.