激动型抗4-1BB抗体3H3交联促进CD8^+ T细胞中Akt激酶活性

Promotion of the activity of Akt in CD8^+ T cells by the crosslink of 3H3,an agonist antibody of 4-1BB

目的:研究4-1BB信号促进CD8+T细胞增殖、存活及Akt活性的变化。方法:利用小鼠CD8α+T细胞磁珠负性分选试剂盒制备高纯度CD8+T细胞,并通过体外抗CD3单克隆抗体(mAb)刺激活化CD8+T细胞。再通过流式细胞术(FCM)分析3H3交联与否,四唑盐(WST-1)/ECS检测CD8+T细胞的增殖程度,Annexin V检测抗凋亡能力,免疫印迹法分析胞内Akt磷酸化水平及Akt激酶活性。结果:3H3交联可以明显促进活化的CD8+T细胞增殖和存活,并且3H3介导的4-1BB信号可明显促进CD8+T细胞内Akt磷酸化水平及Akt激酶活性。结论:Akt途径可能参与了3H3交联介导的4-1BB信号对CD8+T细胞增殖与存活的调节效应。

AIM： To investigate whether 4-1BB could promote the proliferation and survival of CD8^＋ T cells proliferation, and the activity of protein kinase B （Akt）. METHODS： highly purified CD8^＋ T cells were separated and stimulated by CD3 antibody. The crosslink of 3H3 and the proliferation and antiapoptosis of CD8 ^＋ T cells were examined. RESULTS： The crosslink of 3H3 promoted the proliferation and survival of CD8^＋ T cells. 4-1BB induced by 3H3 promoted the phosphorylated level and kinase activity of Akt. CONCLUSION： Akt pathway might participate in 4-1BB which promoted the proliferation and survival of CD8^＋ T cells induced by 3H3.