青藤碱对大鼠心脏移植排斥反应期间ICAM-1和IL-2的影响

The effects of sinomenine on the expresssion of ICAM-1 and IL-2 during the rejection of rat cardiac allograft

目的:观察大鼠同种异位心脏移植排斥反应期间的病理特征,探讨青藤碱对大鼠心脏移植排斥反应的影响及其发生机制。方法:SD健康大鼠为供体,Wistar大鼠为受体,建立大鼠异位心脏移植模型。实验分4组:A组为健康SD大鼠心脏作对照组,B组为SD大鼠到SD大鼠的同基因移植组,C组为SD大鼠到Wistar大鼠异基因移植组,D组为清藤碱治疗组。以ELISA、免疫组织化学检测移植心组织中IL-2和ICAM-1的表达。结果:A、B组未见排斥反应发生,心脏组织中的ICAM-1和IL-2表达水平很低;C组有明显的炎症反应并见大量淋巴细胞浸润,组织中ICAM-1和IL-2的表达水平明显升高(P<0.05);D组炎症反应有所减轻,同时少有淋巴细胞浸润;组织仅微弱表达ICAM-1和IL-2。结论:移植心脏ICAM-1和IL-2的表达水平与排斥反应的发生和发展有关,青藤碱能显著抑制移植心ICAM-1和IL-2的表达和淋巴细胞的浸润,减轻排斥反应,明显延长移植物的存活。

AIM： To observe the pathological characteristics of the rejection of rat cardiac allograft, study the effects and mechanisms of sinomenine on the rejection of rat cardiac allograft. METHODS： The rat model of heterotopic cardiac allograft was established. The rats were randomly divided into four groups： Group A（control group of healthy SD rats）, Group B （cardiac allograft of SD to SD rats）, Group C （cardiac allograft of SD to Wistar rats） and Group D （sinomenine treated group of cardiac allograft of SD to Wistar rats）. ELISA and immunohistochemical staining were carried out to estimate the expression level of IL-2 and ICAM-1 in the grafts. RESULTS： Little ICAM-1 and IL-2 was observed in both Group A and Group B and the infiltration of lymphocytes was not so obvious. In group C, ICAM-1 and IL-2 were increased significantly and the infiltration of lymphocytes was obviously. In group D, inflammatory reac- tion and infiltration of lymphocytes were much less than those in group C, and ICAM-1 and IL-2 is expressed weakly. CONCLUSION： The expression level of ICAM-1 and IL-2 was relevant to the occurrence and development of graft rejection. Sinomenine could obviously reduce the expression of intercellular adhesion molecule and the infiltration of lymphocytes in the grafts and prolong grafts survival signifiantly.