脂肪酸结合蛋白在人乳腺癌组织的表达及意义

Expression of fatty acid binding protein in human breast cancer tissues

目的研究脂肪酸结合蛋白(FABP)mRNA及蛋白质在浸润性乳腺导管癌和乳腺纤维腺瘤的表达及分布,探寻人浸润性乳腺导管癌新的分子标志,为乳腺癌的靶向治疗提供理论依据。方法用半定量逆转录聚合酶链反应、免疫组织化学染色和Western blot等方法检测脂肪细胞型FABP、心型或骨骼肌型FABP、脑型FABP、表皮或牛皮癣型FABP、肝型FABP、小肠型FABP和胃型FABP mRNA及蛋白质在35例浸润性乳腺导管癌,16例乳腺纤维腺瘤组织中的表达变化。结果RT-PCR结果显示A-、B-、I-和G-FABPs mRNA在两种组织的表达差异无统计学意义(P>0.05);但E-、H-和L-FABP mRNA在浸润性导管癌的表达较纤维腺瘤显著升高(P<0.05)。免疫组织化学染色显示,E-、L-和H-FABP在浸润性导管癌的阳性细胞百分数较纤维腺瘤明显上调(P<0.05),分布范围更广。Western blot分析结果进一步证实,E-、L-和H-FABP蛋白质在浸润性导管癌表达明显上调(P<0.05)。结论E-、L-和H-FABP与浸润性乳腺导管癌的发生发展有关,对进一步探寻浸润性乳腺导管癌的分子标志及治疗途径具有理论意义。

AIM： To detect the expression pattern of fatty acid binding protein （FABP） in human breast cancer, and to find potential markers and therapeutic targets for breast cancer. METHODS： adipocyte-FABP （A-FABP）, heart or muscle FABP （ H-FABP）, brain-FABP （ B-FABP）, epidermis or psoriasis FABP （E-FABP）, liver FABP （L-FABP）, intestinal FABP （I-FABP） and gastro-FABP （G-FABP） expression in 35 ductal infiltrating carcinoma and 16 fibroadenoma of breast were detected by RT-PCR, immunohistochemical staining and Western blot analysis, respectively. RESULTS： E-, L-, and H-FABP were up regulated significantly in ductal infiltrating carcinoma when compared with those in benign tissue （ P 〈0.05）. However, there were not significant difference in A-, B-, G-, and I-FABP expression between ductal infiltrating carcinoma and benign tissue （P〉0. 05）. Interestingly, H-FABP was not only found in benign tissues but in some of ductal infiltrating carcinomas, furthermore, H-FABP level was elevated in malignant tissue, compared with that in benign tissue. CONCLUSION： E-, L-, and H-FABP may play a key role in the progress of invasiveness and metastasis in human breast cancer. Furthermore, the secretion of these FABPs has the potential to serve as a diagnostic marker for breast cancer.