西安地区幽门螺杆菌iceA1、iceA2和babA2基因型与致病性的研究

Relationship between iceA1,iceA2 and babA2 genes of Hp in Xi’an and gastroduodenal diseases

目的:检测西安地区幽门螺杆菌(Hp)菌株iceA1,iceA2和babA2基因性分布及其与胃十二指肠疾病的关系。方法:从胃十二指肠疾病患者的胃黏膜活检组织中分离培养到Hp菌株88株,抽提基因组DNA后用PCR法检测Hp的iceA1,iceA2和babA2基因的分布,用χ2检验分析各基因在不同胃十二指肠疾病中的统计学意义。结果:163份标本中检出Hp88株,检出率为54%。iceA1基因性阳性率为76.1%,iceA2为21.6%,babA2为25%,各基因在不同类型疾病中的分布无统计学意义(P>0.05)。结论:西安地区Hp的iceA1占绝大多数,iceA1,iceA2和babA2基因型与Hp感染后的临床结局无相关性。

AIM： To investigate the distribution of iceA1, iceA2 and babA2 genes of helicobacter pylori （Hp） in Xi＇an and analyse the relationship between these genes and gastroduodenal diseases. METHODS： 88 Hp strains were isolated from the patients with different gastric duodenal diseases. After genomic DNA was extracted, iceA1, iceA2 and babA2 genotypes of Hp were determined by polymerase chain reaction （PCR）. The results were analyzed statistically by Х^2 test. RESULTS： 88 Hp strains were detected from 163 samples and the detection rate was 54%. The positive rate of iceA1, iceA2 and babA2 of the 88 Hp strains was 76.1%, 21.6% and 25%, respectively. These genotypes showed no statistical difference in the distribution of different gastric duodenal diseases （ P 〉 0. 05）. CONCLUSION： iceA1 of Hp ispredominated in Xi＇an area. These genotypes have no correlation with the clinical outcome of Hp infection.