摘要
分离自土壤的4个细菌菌株对小麦纹枯病菌具有较强的颉抗作用,平板抑制率达80%以上.采用生防细菌发酵液处理小麦种子,室内盆栽人工接种试验,生防细菌B296、B253对小麦纹枯病的防效分别是65.5%和41.9%.对B296进行抗药性标记后处理小麦种子,研究其在小麦根际的定殖特性,结果发现B296的数量随小麦生长呈下降趋势,在自然土中下降速度比灭菌土更快.
Four bacteria isolates B296, B253, B100, B5 isolated from the field soil showed higher antagonistic effect against Rhizoctonia cerealis, the inhibitory rates were all over 80 % in vitro. Wheat seed were dipped in ferment liquid products and then planted in pots as well as pathogen was inoculated in soil, the control effect of isolate B296 and B253 were 65.5 % and 41.9 % respectively. The colonization of B296 was studied. By treating wheat seed using B296 with the antibiotic marker. The result showed that the number of B296 decreased in the rhizosphere soil and decreased more obviously in field soil than in sterile soil with the grouth of wheat.
出处
《河南科学》
2007年第3期420-422,共3页
Henan Science
基金
国家粮食丰产科技工程河南课题(2004BA520A06-11)
河南省自然科学基金研究项目资助(0311030800)
关键词
小麦纹枯病
细菌
筛选
定殖
生物防治
wheat sharp eyespot
bacterium
screening
colonization
biological control
