3'-大豆苷元磺酸钠抗实验性小鼠良性前列腺增生的作用观察

Antagonistic Effect of 3'-Daidzein Sulfonate Sodium on Prostatic Hyperplasia in Mice

目的:研究3'-大豆苷元磺酸钠(DSS)对实验性小鼠BPH及性激素平衡的影响。方法:40只雄性小鼠随机分为5组:正常对照组、BPH模型组、前列康组(阳性对照)、20mg/(kg.d)DSS组及40mg/(kg.d)DSS组,每组8只。皮下注射丙酸睾酮建立小鼠BPH模型。正常对照组给予橄榄油0.1ml/只,前列康组给予前列康5g/(kg.d),DSS组给予DSS20mg、40mg/(kg.d)灌胃。观察每组处理12d后小鼠前列腺湿重、前列腺指数、前列腺组织形态学变化和性激素水平。结果:DSS治疗12d后,与模型组相比,DSS组小鼠前列腺湿重及前列腺指数出现剂量依赖性的降低(P(0.05或P(0.01),光镜下见增生的腺上皮乳头减少或消失,腺体上皮细胞呈低立方或扁平状;血清T、E2含量和T/E2比值明显降低(P(0.05或P(0.01),40mg/(kg.d)DSS组的结果与前列康组相似。结论:DSS对丙酸睾酮所致小鼠BPH具有显著的拮抗作用,其作用机制在一定程度上与降低小鼠血清T、E2含量及T/E2比值有关。

Objective： To study the antagonistic effect of 3-daidzein sulfonate sodium （DSS） on benign prostatic hyperplasia（BPH） and its possible mechanism. Methods：Forty healthy mice were randomly divided into five groups ： a normal control group without any treatment, a model group of BPH treated by subcutaneous injection of testosterone propionate, a positive control group with the BPH procedure treated by Qianliekang, a 20 mg/（kg· d） DSS group with the BPH procedure and a 40 mg/（ kg · d） DSS group with the BPH procedure. After 12 days of the above treatments, the mice were sacrificed for measurement of the prostate glandular wet weight, the index of prostate gland（ PI）, the morphological changes of prostate gland by light microscopy and the contents of testosterone and estradiol in the serum. Results ： The prostate wet weight and PI decreased dose-dependently after DSS treatment for 12 days compared with the BPH model group（P 〈 0.05 or P 〈 0.01 ）. The hyperplastic epithelioglandular papilla waned and even disappeared in the DSS treated groups under the light microscope, the epithelial cells became cubical or flat. The effect of DSS at 40 mg/（ kg · d） was similar to that of the positive anti-BPH drug Qianliekang. DSS reduced the serum testosterone, estradiol contents and the T/E2 ratio（P 〈0.05 or P 〈0.01）. Conclusion： DSS has significant antagonistic effect on BPH induced by testosterone propionate in mice, which may involve its regulatory action on the sex hormone balance.