灵芝孢子粉对2型糖尿病大鼠附睾细胞线粒体Ca^(2+)细胞色素C的影响

Effects of Ganoderma Lucidum Spores on Mitochondrial Calcium Ion and Cytochrome C in Epididymal Cells of Type 2 Diabetes Rats

目的:研究灵芝孢子粉对2型糖尿病大鼠附睾组织中细胞色素C(Cyt-C)、线粒体Ca2+的影响。方法:青春期Wistar大鼠50只,大鼠尾静脉一次性注射2%链脲佐菌素(STZ)或柠檬酸-柠檬酸钠缓冲液制备2型糖尿病大鼠模型和对照组模型。糖尿病大鼠模型随机分模型组和灵芝组,每组20只,分别给以高脂高糖饮食、高脂高糖+灵芝孢子粉[250mg/(kg.d)],对照组10只,正常饮食。10周后,取双侧附睾,检测附睾细胞线粒体Ca2+、Cyt-C含量。结果:2型糖尿病模型制备成功,模型组附睾细胞线粒体Ca2+含量[(3.279±0.502)mg/L]明显高于对照组[(2.606±0.048)mg/L,P<0.01],灵芝组[(2.693±0.196)mg/L]明显低于模型组(P<0.05),与对照组差异无显著性(P>0.05)。模型组线粒体Cyt-C含量[(3.213±1.511)μmol/L]明显少于对照组[(5.688±1.679)μmol/L,P<0.05],胞质Cyt-C含量[(2.484±0.661)μmol/L]明显高于对照组[(1.574±0.329)μmol/L,P<0.01];灵芝组线粒体Cyt-C含量[(5.258±1.560)μmol/L]高于模型组,但差异无显著性(P>0.05),胞质Cyt-C含量[(1.727±0.396μmol/L]明显低于模型组(P<0.05),与对照组差异无显著性(P>0.05)。结论:2型糖尿病大鼠附睾细胞钙稳态失衡、线粒体有损伤,附睾细胞可能存在细胞凋亡过度。灵芝孢子粉在糖尿病状态下,对附睾组织有保护作用或有对抗细胞凋亡作用。

Objective： To observe the effects of ganoderma lucidum spores （GLS） on mitochondrial calcium ion and cytochrome C in the epididymal cells of type 2 diabetes rats. Methods ： Fifty adolescent rats were randomly divided into a model group （ n = 20）, a GLS group （n =20） and a control group （n = 10）. The animals of the former two groups were injected with 2% STZ via vena caudalis for one time to induce type 2 diabetes. Then the model group was given high-fat-sugar diet, the GLS group high-fat-sugar diet ＋ GLS （250 mg/kg ·d）, and the control group normal diet ＋ CA-citrate sodium buffer. The bilateral epididymides were obtained 10 weeks later and the contents of mitochondrial calcium and cytochrome C detected. Results： Type 2 diabetes models were successfully constructed. The content of mitochondrial calcium in the epididymal cells was significantly higher in the model group （ [ 3. 279± 0. 502 ] mg/L） than in the control group （ [ 2. 606 ±0. 048 ] mg/L, P 〈 0.01 ）, with no significant difference between the GLS group （ [ 2. 693 ±0. 196 ] mg/L） and the control （P 〉 0.05 ）. In the model group, the content of mitochondrial cytochrome C （ [ 3. 213 ± 1.511 ] μmol/L） was significantly lower （ P 〈 0.05 ） while that of cytoplasm cytochrome C （ [ 2. 484 ± 0. 661 ]μmol/L） significantly higher （P 〈 0.05） than in the control （ [ 5. 688 ±1. 679 ]μmol/L and [ 1. 574 ±0. 329 ] μmol/L, respectively）. In the GLS group, the content of mitochondrial cytochrome C （[5. 258 ± 1. 560]μmol/L） was higher, with no significant difference （P 〉 0.05 ）, and that of cytoplasm cytochrome C （ [ 1. 727 ± 0. 396 ] μmol/L） significantly lower than in the model group （ P 〈 0.05 ）, but the difference between the GLS and the control group was not significant （P 〉 0. 05）. Conclusion ： With disequilibrium of calcium homeostasis and damage to mitochondria, there might be excessive apoptosis in the epididymal cells of type 2 diabetes rats. Ganoderma lucidum spores could protect epididymal cells and counteract their apoptosis in diabetic condition.