硝苯地平对人精子膜功能完整性及压力敏感性Ca^(2+)内流影响的实验研究

Nifedipine Modifies Plasma Membrane Functional Integrity and Inhibits Osmosensitive Calcium Influx in Human Sperm

目的:探讨模拟正常血药浓度下硝苯地平体外对人精子膜功能完整性及压力敏感性Ca2+内流的影响。方法:将10例生育男性的精液经上游优化处理后,分别与20、100、20×103ng/ml的硝苯地平在体外共同孵育60min,同时设立对照组进行低渗肿胀(HOS)试验及测定压力导致的精子细胞内钙离子浓度的变化。结果:20×103ng/ml组的精子HOS百分率明显低于对照组,差异具有显著性(P<0.01)。20、100、20×103ng/ml组的精子细胞内Ca2+荧光差值均明显低于对照组,差异具有显著性(P<0.01)。结论:硝苯地平在体外可以改变精子膜功能完整性,抑制压力敏感性Ca2+内流。治疗剂量的硝苯地平在体外可影响精子质量。

Objective： To investigate the effect of nifedipine of therapeutic dosage on the plasma membrane functional integrity and osmosensitive calcium influx in human sperm in vitro. Methods： Sperm samples were aseptically obtained from 10 healthy fertile men by masturbation and prepared by swim-up technique to produce a spermatozoal solution of high motility, The solution was then incubated with nifedipine of 20, 100 and 20 × 10^3ng/ml respectively at 37 ℃in vitro. The hypo-osmotic swelling （HOS） test was done to assess the sperm function. Intracellular calcium concentration was measured by fluorescent probe fura-2/AM before and after sperm medium dilution in distilled water. Results： The 20 ×10^3 ng/ml group showed significantly lower HOS scores than the control （P 〈 0.01 ）. The 20, 100 and 20 × 10^3ng/ml groups all showed significantly lower Ca^2＋ fluorescence D-value than the control （ P 〈0.01 ）. Conclusion ： Nifedipine can modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm and affect male fertility in vitro in therapeutic dose.