紫外线致成纤维细胞损伤及VitE保护作用的研究

Research on Photodamage of UVB on Fibroblasts in Vitro Culture and Photoprotection of VitE on Cells

目的观察UVB辐射对体外培养的真皮成纤维细胞的损伤作用及VitE对细胞的光保护作用。方法由健康人皮肤中分离培养成纤维细胞,用紫外线以一定时间(1、5、10、15分钟)进行照射,并加入VitE进行干预处理。MTT法检测细胞增殖活性,荧光显微镜检测各受试组细胞凋亡率。结果UVB照射后24h,上述细胞均出现增殖活性下降,活性下降程度与照光时间成正比;加入VitE处理后,细胞活性可有一定程度恢复。UVB照射可诱导真皮成纤维细胞产生凋亡,其凋亡率随UVB照射时间延长而增加。加入VitE处理后,各组HeLa上皮细胞和成纤维细胞凋亡率均明显下降。结论紫外线辐射可引起真皮成纤维细胞损伤,VitE可抑制紫外线辐射的损伤效应。

To study the photodamage of UVB on fibroblasts in vitro cult ure and HeLa cells,and to study the photoprotection of VitE on cells. Methods Fibroblasts（FB） were isolated from human skin samples. Fibroblasts were irradiated with different time of UVB irradiation（ 11,5,10,15 min） and treated With VitE for interi＇erence. MTT assay was used to record cell proliferation and cellular activ-ity. Apoptotic rate were determined with a fluorescent microscopy. Results After 24 hours of UVB expo-sure,cell proliferation and cellular activity was low,and the irradiation damage of skin cells （fibroblasts） was dependent on the irradiated dosages. The：VitE could protect the Cellular activity partly. UVB induced apoptosis of skin fibroblasts cells,and induction was： dose-dependent, VitE showed protective effect on UVB-induced apoptosis and the apoptotic rate was reduced in all irradiation dosages. Conclusions UVB irradiation can inhibit the growth of fibroblasts and HeLa cells and can induce apoptosis, and it is dose-dependent; Such effect Can be antagonized by VitE administration.