毛细管气相色谱法测定止痒灵中的苯酚和薄荷脑

Simultaneous determination of phenol and menthol in itch relief agent by capillary gas chromatography

目的建立毛细管气相色谱法同时测定止痒灵中苯酚和薄荷脑的含量。方法采用HP-5MS（30m×0．32mm×0．25μm）弹性石英毛细管为分离柱，FID检测器，樟脑为内标，在70～150℃程序升温，分流进样。结果苯酚和薄荷脑分别在0．03324～0．3324mg／mL（r=0．9994）、0．03276～0．3276mg／mL（r=0．9999）浓度范围内线性关系良好，苯酚和薄荷脑的平均回收率和RSD分别为99．99％和0．78％、100．9％和0．81％。结论本法消除了止痒灵中甘油的干扰，简便、快速和准确。

Objective To establish a capillary gas chromatography for determining the contents of phenol and menthol in itch relief agent. Methodes The analysis was performed on an HP-5MS elastic quartz capillary （30 m × 0.32 mm × 0.25 μm）. FID was used as detector and camphor as internal standard. Phenol and menthol were simultaneously analyzed under temperature programming in 70 - 150℃ and split stream sampling. Resufls The calibration curves were linear in the ranges of 0. 03324 - 0. 3324 mg/mL for phenol （ r = 0.9994）, 0.03276 - 0. 3276 mg/mL for menthol （ r = 0. 9999 ） . The average recoveries were 99.99% （ RSD 0. 78% ） for phenol, 100.9% （ RSD 0.81% ）for menthol. Conclusion The method is sample, fast and accurate, which eliminates the disturbance of glycerin in the agent.