醋酸棉酚对雄性小鼠生育能力影响的实验研究

A Study on the effects of gossypol acetate on fertility of male mice

目的探索醋酸棉酚对雄鼠生殖系统的影响,为生态灭鼠药的研制提供实验依据。方法将雄性小鼠随机分成对照组和实验组,每组10只,通过灌胃的方法,对照组灌生理盐水,实验组按50、120、200 mg/kg体质量浓度灌胃,分别于灌胃10 d、20 d后解剖,取睾丸、附睾,进行精子细胞观察、精子计数、精子活率和精子形态观察,如指标有变化,进行交配试验检测醋酸棉酚对小鼠生育能力的影响。结果灌胃10 d各指标组间差异均无显著性;20 d后,200 mg/kg组与对照组精子数目和睾丸系数变化差异有显著性(P<0.05),睾丸切片可见120 mg/kg和200 mg/kg组生精小管的初级精母细胞与精原细胞或基膜之间有较大的空隙,部分生精小管腔内的精子尾部减少。合笼后产子数量和怀孕率对照组与120 mg/kg组和200 mg/kg组差异有显著性(P<0.05),120 mg/kg组和200 mg/kg组间差异无显著性(P>0.05)。结论醋酸棉酚对雄鼠生殖系统的影响可能与其浓度和药物作用的时间有关。50 mg/kg组在20 d内对小白鼠生育能力没有影响,120 mg/kg和200 mg/kg组在20 d内对小白鼠生育能力有影响。

Objective To investigate the effect of gossypol acetate on reproductive systems of male mice, providing useful data for development of environment-friendly rat poison. Methods Male mice were randomly assigned into two groups, with 10 mice in each group. Mice in control group were administered intragastrically with the physiological saline, and mice in the experimental groups were administered intragastrically with gossypol acetate at the dose of 50,120,200 mg/kg. Epididymis were taken to observe the sperm counts, motility rate and the sperm shape after 10 days and 20 days respectively. In the ease that diversified parameters were observed, mating test was conducted to detect the effects of gossypol acetate on fertility of male mice. Results There were no statistics significance between the experimental group and control group after 10 days, however, sperm counts and testicle/body weight indices had statistics significance （ P 〈 0.05 ） after 20 days. Thurdermore, there were visible difference between the groups administered with gossypol acetate at the doses of 120,200 mg/kg and the control group in testicle section observation with microscopy. After having the birth ability test, number of baby mouse and pregnancy rates have statistic significance in the groups administered with gossypol acetate at the doses of 120 and 2 00 mg/kg groups. Conclusions The effect of gossypol acetate was possibly concerned with its concentration and the administration time. There was no effect in the group administered with gossypol acetate at the dose of 50 mg/kg in 20 days, but not in the group administered with gossypol acetate at the doses of 120 mg/kg and 200 mg/kg.