肉用绵羊2号染色体GDF8区域4种微卫星标记多态性研究

Study on Four Microsatellite DNA Polymorphism in the Region of GDF8 on Chromosome 2 in Meat Sheep Populations

选择了2号染色体GDF8区域可能与生产性能相关性较大的4个高度多态微卫星基因座(BM81124,TEXAN2,FCB128,BMS1591),分析这些位点在Dorset、Suffolk和Texel3个肉羊品种的多态分布情况。结果表明,这4个微卫星标记在3个绵羊品种中的等位基因数分别为:10、12、7、11,其多态信息含量/杂合度分别为:0.7778/0.8045、0.712/0.7520、0.7551/0.7852、0.8057/0.8289,可见这些微卫星位点在3个绵羊品种中具有比较丰富的多态性。因此它们可以作为进行生产性状与标记间连锁分析的遗传标记,用于进一步的QTL定位研究。

According to report, four highly polymorphic microsatellite loci possibly linked to production traits and mapped to the region of GDF8 on sheep chromosome 2 were selected, they were BM81124, TEXAN2 ,FCB128,BMS1591. They were analyzed for polymorphism in Dorset, Suffolk and Texel meat sheep populations. Results showed that the number of alleles were 10,12,7,11 and polymorphic information content / Heterozygosity were 0. 7778/0. 8045,0. 712/0. 7520,0. 7551/0. 7852, 0. 8057/0. 8289. We can see these microsatellite loci have rich polymorphism in three groups. So they can be used as the linkage analyse between the production traits and makers,and used for further location of QTL.