婴幼儿血镉含量与MDA含量和SOD活力的关系

Effects of Blood Cadmium Level on Content of MDA and Activities of SOD of Babies

目的从临床角度研究婴幼儿血镉水平及与MDA和SOD的关系。方法收集48名婴幼儿静脉血,采用原子吸收光谱仪测定血镉的含量,硫代巴比妥法测定血液中的丙二醛(MDA)含量,黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力。按血镉水平将48名受试者分为正常组、低镉组、高镉组。结果正常组血液中MDA含量为(6.57±5.00)μmol/L;低镉组、高镉组分别为(11.59±7.92)μmol/L和(15.45±7.32)μmol/L,与正常组比较,差异均有统计学意义(P<0.05,P<0.01),并与血镉浓度呈显著正相关(r=0.53,P<0.01)。血液中SOD含量正常组为(93.44±14.28)U/ml;低镉组、高镉组分别为(80.03±16.09)U/ml和(72.35±19.88)U/ml,与正常组相比,差异均有统计学意义,(P<0.05,P<0.01),并与血镉浓度呈显著负相关(r=-.052,P<0.01)。结论血液中MDA含量或SOD活力的变化可作为临床上判断婴幼儿期镉损伤的重要指标之一。

Objective To clinically examine the damage of malondialdehyde （MDA）and superoxide diamutase（SOD）activities of babies induced by blood cadmium. Methods The blood cadmium of 48 babies was measured with AAS; the content of MDA was determined with thiobaritone method, and SOD with xanthosine oxidase method. The babies were divided into three groups, i.e. normal group, low cadmium group, and high cadmium group according to the blood cadmium levels. Results The contents of MDA in normal group（6.57±5.00）μmol/L, and in low cadmium group and high cadmium group were（11. 59±7.92） μmol/L and（15.45±7.32）μmol/L respectively. Compared with the normal group, the contents of MDA in the low cadmium group and high cadmium group were higher with significant differences （P〈0. 05 and P〈0. 01 respectively）, and there was a distinctive positive correlation（r= 0. 53,P〈0. 01）. The contents of SOD in the normal group were （93.44±14.28）U/ml, and in low cadmium group and high cadmium group were （80.03±16.09） U/ml and （72.35 ±19.88）U/ml. Compared with the normal group, the contents of SOD in the low cadmium group and high cadmium group were lower with significant differences （P〈0.05 and P〈0.01 respectively） showing distinctive negative correlation （r=-0. 52,P〈0. 01）. Conclusions The contents of SOD and MDA in blood might be the important clinical damage indicators induced by cadmium in babies.