用尿沉淀细胞DNA甲基化状态的分析检测膀胱癌

Detection of bladder cancer by accessing DNA methylation state in urine sediments

目的:确定尿沉淀细胞DNA中的13个肿瘤相关基因启动子的甲基化谱式分析在膀胱癌诊断中的价值。方法:用定性甲基化特异性(methylation specific polymerase chain reaction,MSP)的方法,对92例临床确诊的膀胱癌患者、23例非肿瘤性尿路疾病患者、6例脑外科患者、7例健康志愿者检测了尿沉淀细胞DNA中肿瘤相关基因启动子的甲基化状态。结果:在临床确诊的92例膀胱癌患者中被检测的13个基因的高甲基化状态出现频率显著高于23例非肿瘤性尿路疾病患者,差异有统计学意义(P<0.05)。而6例脑外科患者和7例正常健康人的尿沉淀细胞DNA中,上述基因均为去甲基化状态。若以任一个基因高甲基化为膀胱癌的指征,88.0%(81/92例)的膀胱癌可被检出。结论:MSP法分析尿沉淀细胞DNA中肿瘤相关基因启动子的甲基化状态可有效地检出膀胱癌。

Objectives：To assess DNA methylation state of the 13 genes in urine sediments and evaluate their value in diagnosis of bladder cancer. Methods：Ninety-two patients with bladder cancer, 23 patients with non tumorous urinary disease, 6 patients with neurological disease, and 7 healthy volunteers were recruited in the study. The DNA methylation state of tumor-related promoters in urine sediments was determined by Methylation specific PCR method （MSP）. Results： The frequency of hypermethylation of 13 genes in 92 eases with bladder cancer was higher than that in 23 eases of bladder diseases,while all the genes were at demethylation state in the 7 healthy volunteers and 6 patients with neurological disease. The incidence of hypermethylation was significantly higher in bladder cancer compared with non tumorous urinary disease （P 〈0.05）. If DNA hypermethylation was used as a positive indicator, 88.0% （81/92eases） of bladder cancer could be detected. Conclusion： Assessing DNA methylation state in the promoter of tumor-related genes in urine sediments by MSP method is effective for detection of bladder cancer.