摘要
目的:采用人牙齿切片体外器官培养的方法研究L型钙离子通道α1亚基D亚型多克隆抗体对牙本质形成的影响。方法:收集年轻人健康前磨牙,用慢速切锯制备2 mm厚的牙齿横切片,将在L型钙离子通道α1亚基D亚型多克隆抗体中浸泡过的琼脂糖微球与在PBS液中浸泡过的琼脂糖微球对称放置在牙片上,将切片用含琼脂糖的半固体培养基包被后采用Trowel器官培养方法培养1周,观察其对牙本质形成和矿化的影响。结果:L型钙离子通道α1亚基D亚型多克隆抗体组四环素线状荧光带较对照组变细,von Kossa染色前期牙本质处球状矿化也较对照组减少,I型胶原免疫组化染色显示实验组与对照组无明显区别。结论:L型钙离子通道α1亚基D亚型多克隆抗体能够影响牙本质的矿化,但对牙本质基质的合成和分泌无明显影响。该结果提示成牙本质细胞膜上的L型钙离子通道α1亚基D亚型对牙本质矿化过程中的钙离子转运有重要作用。
AIM： To study the effects of polyclonal antibody to L type calcium channel α1 D subunit on human dentinogenesis by the method of the slice organ culture of the human tooth in vitro. METHODS ： Young healthy human premolars were collected, and cut into 2mm -thick transverse slices by low speed diamond -edged saw. Agarose beads dipped in polyclonal antibody to L type calcium channel α1 D subunit and PBS were symmetrically placed on tooth slices, and the slices were then embedded in a semisolid agarose - based medium and cultured in Trowel - type cultures for 1 week. Dentinogenesis and calcification of the slices were observed. RESULTS. Fluorescent band of tetracycline in the experimental group was narrow, and globular calcification in predentine was decreased compared with the control group. Immunohistochemical staining for collagen I showed no obvious difference between the two groups. CONCLUSION： Polyclonal antibody to L type calcium channel al D subunit can influence the calcification of dentine, but has no obvious influence on the synthesis and secretion of dentine matrix. It can be concluded that L type calcium channel α1 D subunit may play an important role in calcium transport during dentine calcification.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第5期247-250,共4页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(30471889)
