尖锐湿疣患者皮损中SOCS1和PIAS1的表达及意义

Expression and significance of suppressor of cytokine signaling-1 and protein inhibitor of activated STAT-1 in condyloma acuminatum

目的探讨尖锐湿疣（CA）皮损角质形成细胞中细胞因子信号转导JAK／STAT通路负调控蛋白细胞因子信号抑制剂-1（SOCS1）和活化STAT的蛋白抑制剂-1（PIAS1）的表达。方法采用SP免疫组化染色技术检测40例CA患者皮损、20例宫颈癌和20例正常人皮肤（包皮）中SOCS1和PIAS1的表达及分布。结果①CA患者皮损中SOCS1和PIAS1的表达阳性细胞分布主要位于棘细胞层，为棕黄着色，阳性反应定位胞质；宫颈鳞状细胞癌亦为胞质阳性表达，棕黄着色，呈弥漫性分布；而正常包皮少数阳性着色主要定位于基底层细胞的胞质，呈浅黄着色。②CA皮损中SOCS1和PIAS1的表达阳性率分别为85％和80％，高于在正常人上皮中的表达（阳性率分别为30％和35％），两组比较差异均有统计学意义（前者X^2=18．15，P〈0．01；后者X^2=11．87，P〈0．01）；宫颈癌中SOCS1和PIAS1的表达阳性率分别为90％和85％，虽较CA有升高，但两者比较差异均无统计学意义（两者均P〉0．05）。③CA中SOCS1与PIAS1的表达无显著相关性（rs=0．14，P〉0．05）。结论CA皮损中可能通过细胞因子信号转导负调控蛋白SOCS1和PIAS1在角质形成细胞过度增殖或恶性转化过程中起调控作用。

Objective To explore the expression of suppressor of cytokine signaling-1 （ SOCS1 ） and protein inhibitor of activated STAT-1 （ PIAS1 ） in keratinocytes of condyloma acuminatum （ CA ） lesions and its significance. Methods SP immunohistochemistry technique was used to detect the expression and distribution of SOCS1 and PIAS1 in lesions from 40 cases of CA and 20 cases of cervical cancer, and in skin （foreskins） of 20 normal controls. Results In CA lesions, SOCS1 and PIAS1 were stained brown-yellow in the cytoplasm of keratinocytes, and positive cells were mainly distributed in the prickle layer. In cervical cancer, they were also stained brown-yellow and diffusely distributed in the cytoplasm of carcinoma cells. In normal skin, SOCS1 and PIAS1 weakly expressed in the cytoplasm of keratinocytes in basal layer, and were stained pale yellow. The positive rates of both SOCS1 and PIAS1 were significantly higher in CA lesions than in normal skin （85% vs 30%, X^2 = 18.15, P 〈 0.01; 80% vs 35%, X^2 = 11.87, P 〈 0.01 ）. Although the positive rates of SOCS1 （ 90% ） and PIAS1 （ 85% ） in cervical cancer were higher than those in CA, no significant difference was found between them （ both P 〉 0.05 ）. There was no significant correlation between the expression.of SOCS 1 and PIASI in CA lesions （ rs = 0.14, P 〉 0.05 ）. Conclusion SOCS1 and PIAS1 may play a role in the overproliferation and malignant transformation of keratinocytes in CA lesions.