利用SRAP分子标记划分玉米自交系类群初探

Preliminary Study on the Classification of Maize Inbred Lines by SRAP Marker Technology

以河南省20世纪80年代以来常用的29个玉米自交系为材料,利用SRAP分子标记技术对河南省玉米种质遗传多样性及杂种优势群进行划分,对主要的审定品种亲本间遗传距离及杂种优势模式进行分析。结果表明,29个玉米自交系可分为四大类群,第Ⅱ大类又可分为2个亚群,第Ⅲ大类群又可以分为3个亚群,29个玉米自交系可分为7个亚群,分析结果与系谱来源基本一致。分类后类间平均遗传相似系数均小于类内平均遗传相似系数,同时生产上推广组合的亲本大多来自不同的大类或亚类,SRAP标记的聚类结果是比较合理的,对于玉米杂种优势群的划分具有参考价值。

29 maize inbred lines often utilized in Henan since the 1980＇s were used in this study, for which the genetic diversities and heterotic population were analysized by SRAP molecular markers technology while the heterotic patterns and their parents＇ genetic distances were also studied. The results showed： 29 inbred lines were classified into four groups, of which group Ⅱwas divided into two sub-groups and group Ⅲ was divided into three sub-groups, so the 29 inbred lines could be divided into seven sub-groups, which was consistent with the original pedigree. Average genetic similarity coefficient among groups were smaller than that within groups after classification and popularized parental hybrids most came from different groups or sub-groups. So the SRAP markers technology was reasonable and valuable for classification of maize heterotic population.