桃熟性性状的SSR标记

SSR Makers of Maturity Gene in Peach

以油桃(Prunus persicaL.var.nectarina)品种秦光二号(晚熟)和曙光(早熟)的90株正交F1代为试材,采用SSR分子标记技术和BSA法寻找与桃熟性性状基因紧密连锁的分子标记。对SSR反应体系进行了优化,结果显示,最优的反应体系为Taqpolymerase 0.3μL,MgCl22.5μL,10×PCR buffer2.5μL,dNTP 2.0μL,引物1.2μL,模板DNA 30 ng。研究发现,51对SSR引物在亲本中有多态性的有31对引物,占总引物对的61%,用这31对引物在早熟晚熟混合池中筛选发现,引物对P21在混合池中表现稳定的差异。

F1 plants of the direct cross of ＂ Qinguang2＂ and ＂ Shuguang＂ , two nectarine varieties （Prunus persica L. var. nectarina） was used as materials to screen by SSR and BSA（Bulk segregate analysis） the molecular markers closely linking to maturity gene of nectarine. PCR system was optimized and found the most appropriate system was： Taq polymerase 0.3 μL ,MgCl2 2.5 μL,10×PCR buffer 2.5 μL,dNTP 2.0 μL,primer 1.2 μL, DNA 30 ng. The two parents was selected by 51 primer pairs of SSR, as a result 31 primer pairs of SSR behaved diversity, then the pairs were selected by two pools, one molecular marker linking to precocity and serotinous gene of nectarine were screened from 31 paies SSR primer.