低温对不同西葫芦自交系花粉发芽力的影响

The Effect of Low Temperature on Pollen Germinability of Different Pumpkin Inbred Lines

在不同开花温度(FT)和发芽温度(GT)组合处理下,测定了西葫芦14个自交系的花粉发芽率。结果表明:①随FT和GT的降低,西葫芦花粉发芽力下降。正常GT24℃下平均花粉发芽率在48%以上,GT15℃下为30%左右,GT9℃下发芽率极低,接近零,FT和GT的影响都达到极显著,但GT影响较大,FT和GT交互作用不显著。②不同的FT和GT组合下,自交系间花粉的发芽率除FT15℃/GT24℃下差异不显著,其他FT(15℃、12℃、9℃)和GT(24℃、18℃、15℃、12℃、9℃)的各组合温度下,都达到了极显著差异。③在FT9℃/GT12℃培养时,自交系间方差分析的F值最大,且变异系数最大,花粉发芽力的差异达到最大,说明该温度组合可作为鉴定西葫芦不同品系(种)耐冷性的适宜花粉培养温度。

In this experiment, the pollen germination rates of 14 pumpkin inbred lines were mensurated under the different flowering temperature （FT） and germination temperature （GT）. The result Showed that： ①the pollen germinability was going down following the falling of FT and GT, pollen germination rate is above 48% under normal GT24℃, about 30% under GT15℃, the germination rate under GT9℃ is fearfully low, approaching zero, all the influence of FT and GT is highly significant, but, the effect of the GT is bigger, the interaction between FT and GT is not significant.②The difference of the pollen germination rates of different inbred lines is highly significant under the combination between FT（15℃, 12℃, 9℃） and GT（24℃, 18℃, 15℃, 12℃, 9℃） excepting FT15℃/ GT24℃. （3）Cultured under FTg℃/GT12℃, the F value of variance analysis and the coefficient of variation is the biggest, the variance is the biggest for the pollen germinability of different lines, so that, the proper temperature is FTg℃/GT12℃,which will be used to identify the cold tolerance of different pumpkin lines（varieties） by means of pollen germination.