摘要
采用苯酚硫酸法,对青钱柳叶多糖的水提取法、提取工艺以及不同采集期的多糖含量进行了研究.结果表明,水提法的精密度RSD%为2.89%,重现性RSD%值为1.72%,稳定性RSD%值为3.1%;通过L9(3^4)正交试验得出水提法提取青钱柳多糖的最佳提取时间为7h,料液比为1∶20,pH 9;青钱柳叶在5~8月的多糖含量为2.19%.
Water extraction, extract technology and content of different harvest time of polysaccharide from Cyclocarya paliurus (Batal) Ijinskaja (C. Paliurus) Leaves was studied by phenol-sulfuric acid method. Results showed that precision repeatability and stability RSD% of water extraction is 2.89 % 1.72% and 3.1%, respectively; The optimum process conditions (70 minutes extracting time, 1 : 20 solid to liquid ratio and at pH 9)were established by L9 (3^4)orthogonal experiments; Content of C. Paliurus polysaccharide is 2.19% at May and August.
出处
《西北农业学报》
CAS
CSCD
北大核心
2007年第3期192-195,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家科技部星火计划项目(2002EA730001)
江西省科技厅重点项目(赣科发计字2002163)
关键词
青钱柳
多糖
水提法
采集期
Cyclocarya paliurus (Batal) Ijinskaja
Polysaccharide
Water extraction
Harvest time