血管内皮生长因子拮抗内皮细胞凋亡的实验研究

Experimental study of vascular endothelial growth factor on inhibiting the apoptosis of vascular endothelial cell

目的:研究血管内皮生长因子(VEGF)对血管内皮细胞凋亡及凋亡相关基因Bcl-2与apo-1/Fas mRNA表达的影响。方法:将体外培养的人脐静脉内皮细胞(HUVEC)分成对照组、肿瘤坏死因子-α(TNF-α)处理组、TNF-α加VEGF处理组;采用原位末端标记法、流式细胞术观察各组细胞的凋亡发生情况,通过RT-PCR法观察各组细胞中凋亡相关基因Bcl-2与apo-1/Fas mRNA表达的变化。结果:TNF-α处理组凋亡细胞和apo-1/Fas mRNA的表达明显高于对照组和TNF-α加VEGF处理组,而Bcl-2 mRNA表达情况相反。结论:VEGF能拮抗TNF-α诱导的内皮细胞凋亡,其抗凋亡的机制可能与其上调Bcl-2 mRNA表达与下调apo-1/Fas mRNA表达有关。

Objective：To evaluate the mechanisms of vascular endothelial growth factor （VEGF） on prevention of restenosis after percutaneous coronary intervention （PCI） by observing the effect of VEGF on the apoptosis of vascular endothelial cell induced by tumor necrosis factor-α （TNF-α）. Method： Human umbilical vein endothelial cells （HUVEC） were divided into control group, TNF-α-treated group and TNF-α＋ VEGF-treated group, the apoptosis of HUVEC was determined by flow cytometry （FCM） and in situ terminal deoxynucleotidyl transferase （TdT）-mediated deoxyuridine triphosphate （dUTP）-biotin nick end-labeling （TUNEL）, the expression of Bcl-2 mRNA and Fas mRNA were examined by reverse transcription polymerase chain reaction（RT-PCR）. Result： Com- pared with control group and TNF-α＋ VEGF-treated group, the apoptosis cells and the expression of apo-1/Fas mRNA were significantly increased of TNF-α-treated group, but the expression of Bcl-2 mRNA was markedly decreased, and VEGF could inhibit the apoptotic effect of TNF-α on HUVEC. Conclusion： VEGF could inhibit the apoptosis of HUVEC induced by TNF-α which may correlated with upregulation of Bcl-2 mRNA expression and inhibiting Fas mRNA expression.