少血供兔VX2肝移植瘤模型动脉栓塞后影像与病理分析

Comparison of pathology and imaging of hypovascular rabbit liver VX2 tumor after transcatheter arterial chemoembolization

目的建立并筛选少血供兔VX2移植性肝癌模型,观察肝动脉化疗栓塞治疗效果及经肝动脉注射碘油沉积情况。方法开腹种植转移瘤于35只新西兰大白兔肝叶,移植成功后经筛选出少血供移植瘤16只,随机分为2组,对照组8只,插管至腹腔干;实验组8只,插管至肝固有动脉或左肝动脉,术后1周进行CT扫描,观察碘油沉积情况,分别取肿瘤进行HE染色及检测VEGF蛋白表达。结果16只少血供VX2移植瘤模型兔插管成功11只,5只失败。对照组成功者5只插管至腹腔干,实验组6只插管至左肝动脉或肝固有动脉,造影发现肿瘤血管稀疏或不可显示,术后CT扫描发现实验组碘油在瘤内呈环形沉积;对照组5只肝移植瘤中VEGF表达阳性2例,弱阳性3例;实验组6只移植瘤中强阳性4例,阳性2例。结论兔肝动脉血管解剖与人相似,超选择至左肝动脉,暂时阻断肿瘤动脉血供后栓塞化疗,对少血供肿瘤治疗效果较好。CT对术后碘油沉积情况检测方便,可靠,有特征性。

Objective To establish a stable transplanted model of hypovascular rabbit liver vx2 tumor, evaluate the antitumor effects of Iodized oil and 5-fluorouracil via hepatic artery on hypovascular rabbit liver VX2 tumor, and investigate the distribution of Iodized oil in rabbit liver VX2 tumor with CT. Methods Thirty-five white rabbits of New Zealand were included in the study. Xixteen rabbits with hypovascular liver VX2 tumor were divided into two groups at random. In the first group, the mixture of 1 ml Iodized oil and 5-fluorouracil were infused via common hepatic artery into rabbit liver; For the second group, the mixture of lml Iodized oil and 5-fluorouracil were infused via hepatic artery or prop hepatic artery into rabbit liver. Spiral CT scan were performed to observe the distribution of Iodized oil. Rabbits survived seven days after treatment were sacrificed; tumor tissues were obtained and examined histologically. Results The success rate of transcatheter arterial chemoembolization for hypovascular rabbits VX2 liver tumor was 68.7%（11/16）. Eight cases were introduced to the prop hepatic artery or the common hepatic artery, three cases were introduced to the left hepatic artery, tumor staining could not be found on angiography. Iodized oil deposit in the periphery of tumor. The expression of VEGF were enhanced in the Iodized oil group. Conclusion The liver artery of rabbit is similar with humans. Curative effects which were catheterized left hepatic artery. CT scan is a dependable method for monitoring the distribution of iodized oil in tumor.