IBV广东地方株D41S1基因PCR产物的酶切分析及其意义

RESTRICTION ENZYMES ANALYSIS OF IBV GUANGDONG LOGICAL STRAIN D41 S1 GENE PCR PRODUCT AND ITS SIGNIFICANCE

对广东肾变病型鸡中分离致弱的ＩＢＶＤ４１株Ｓ１基因ＰＣＲ产物作了ＨａｅⅢ酶切分析，发现其酶切产物与ＩＢＶＭ４１的一样，而与美国肾变型标准株Ｇｒａｙ、Ｈｏｌｔｅ不同。根据国外用ＲＦＬＰ区分ＩＢＶ血清型的判定标准，Ｄ４１株应归属于马萨诸塞血清型；综合此分离株的其它特性。

Using Hae Ⅲ to digest the S1 gene PCR product of IBV D41 strain isolated from a chicken nephrosis case in Guangdong province, it was found that the digested products were the same as that of IBV M41 strain, and different from that of the American standard nephritic strains Gray and Holte. So the IBV D41 belongs to the Massachusetts serotype, and the IBV strains in South China seem to have undergone mutation.