摘要
对广东肾变病型鸡中分离致弱的IBVD41株S1基因PCR产物作了HaeⅢ酶切分析,发现其酶切产物与IBVM41的一样,而与美国肾变型标准株Gray、Holte不同。根据国外用RFLP区分IBV血清型的判定标准,D41株应归属于马萨诸塞血清型;综合此分离株的其它特性。
Using Hae Ⅲ to digest the S1 gene PCR product of IBV D41 strain isolated from a chicken nephrosis case in Guangdong province, it was found that the digested products were the same as that of IBV M41 strain, and different from that of the American standard nephritic strains Gray and Holte. So the IBV D41 belongs to the Massachusetts serotype, and the IBV strains in South China seem to have undergone mutation.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1997年第1期62-64,共3页
Journal of South China Agricultural University
基金
高校博士点科研基金
国家自然科学基金
关键词
BV
PCR
酶切分析
IBV
PCR
restriction enzyme analysis