Cl^-和Ca^(2+)对亚硝酸盐氮作用于草鱼种毒性的影响

EFFECTS OF CHLORIDE AND CALCIUM ON TOXICITY OF NITRITE TO JUVENILE GRASS CARP

通过对血红蛋白、高铁血红蛋白的测定,研究亚硝酸盐氮对草鱼种的毒害作用,以及对其毒性的影响和中毒后草鱼种的恢复。结果表明,亚硝酸盐氮能将草鱼血液中的血红蛋白转化成高铁血红蛋白,且高铁血红蛋白含量随环境中亚硝酸盐氮浓度的增加呈指数增加;Cl^-、Ca^(2+)均能够抑制高铁血红蛋白含量的升高,显示出一定的保护作用,且二者能产生迭加保护作用。中毒后的草鱼种转入清水中,有明显的恢复现象。

Hemoglobin, methemoglobin levels of juvenile grass carp (Ctenopharyngodon idellus) exposed to nitrite were measured in order to evaluate the following 3 aspects (1) the toxicity of nitrite to juvenile grass carp, (2) the effects of chloride and calcium on the toxicity of nitrite to juvenile grass carp, (3) recovery of nitrite-toxic juvenile grass carp. The results showed that nitrite was able to oxidize hemoglobin to methemoglobin, whose level would increase exponentially with nitrite concentration; protective ability was showed through inhibiting the increasing of methemoglobin content which was by nitrite when Chloride and Calcium were added respectively, especially when they were added together, protective ability was coordinated. Nitriteexposed juvenile grass carp could recover gradually to rome extent after it was transferred to clear water.