马尾松插穗内源抑制物质的研究

STUDY ON THE ENDOGENOUS INHIBITORS IN MASSON PINE (PINUS MASSONIANA LAMB.) CUTTINGS

用浸渍法、萃取法和高效液相色谱法（ＨＰＬＣ）对来源于不同年龄、不同繁殖方式和不同种源马尾松插条母株的插穗内源抑制物质（ＭＩｎ）进行分离提纯。对ＭＩｎ作了初步定性和生物鉴定。用ＨＰＬＣ自动积分仪法比较了各组分含量。研究结果发现ＭＩｎ都含七种组分，它们均属黄酮类和苯酚类物质。随母株年龄递增，ＭＩｎ含量均升高。扦插起源母株ＭＩｎ含量比实生的低。南亚热带种源ＭＩｎ含量比北亚热带的低。ＭＩｎ各组分抑制小麦胚芽鞘伸长程度与其含量高低一致。插穗生根成活率与ＭＩｎ含量呈负相关倾向。ＭＩｎ含量随母株年龄递增是马尾松扦插繁殖年龄效应原因之一。反复扦插可延缓马尾松母株老化。不同种源ＭＩｎ差异意味着开展马尾松无性系选育的可靠性和有效性。阻遏控制ＭＩｎ的基因表达。

The endogeneous in hibitors in masson pine (Pinus massoniana Lamb) cuttings (MIn) from the ortets with different ages,from different provennanes,and by different reproductive modes were purified and separated by the methods of extraction and high pressure liqid chromatography (HPLC).The properties of the MIn were preliminary proofed by means of chemical detection and ultraviolet absorption spectra followed by biological assay with straight growth of wheat coleoptile section.It was found that there were 7 components in MIn in every kind of cuttings,which were flavones and phenols.Thesurvival percentage of each kind of cuttings negatively correlated with their MIn contents.The contents of MIn from different cuttings were compared by HPLC auto-integrator.The MIn content in cuttings from seedling ortets was higher than that from cutting ortets.MIn contents in the ortets from the southern subtropical provenance were lower than those from the northern subtropic provenances.The difference between provenances implied that the clone selective breeding of masson pine would be reliable and effective.The MIn contents increased with the age increasing of the ortets,which was one of the causes of cyclophysis in masson pine cuttings.Masson pine ortets could be rejuvenated by serial cuttage.Stopping the express of the MIn genes would be the successful way for increasing the cutting survival percentage.