RP-HPLP法测定果脯中甘草苷和甘草酸的含量

Determination of Liquiritigenin Contents and Glycyrrhizic Acid in Preserved Fruit by RP-HPLC

目的:建立用RP-HPLC法同时测定果脯中甘草苷和甘草酸含量的方法。方法:采用LiChrospher RP- C_(18)(250mm×4．6mm,i．d．5μm)色谱柱,以乙腈-水(1．0%甲酸)为流动相,梯度洗脱,双波长检测。结果:甘草苷在0．21～6．30μg范围内呈良好的线性关系,回归方程为Y=2334．3161X+25．2431(r=0．9999),平均回收率为98．6%,RSD为1．36%(n=9),重复性RSD为0．78%;甘草酸在0．25～7．50μg范围内呈良好的线性关系,回归方程为Y=907．7837X+0．8969(r=0．9999),平均回收率为100．2%,RSD为1．48%(n=9),重复性RSD为1．25%。结论:该方法简便迅速,结果可靠,分离效果好,可作为控制食品中甘草限量的方法。

Object： To establish a RP-HPLC method for the determination of liquidtin and glycyrrhizic acid in preserved fruit.Methods A LiChrospher RP-C18（250 mm×4.6mm, i.d. 5μIn） column was used and the mobile phase was acetonitrile-water （1.0% methanoic acid） for gradient elution. The double detection wavelengths were used. Results The linear range of liquiritin is 0.21-6.30 μg and regression equation is Y=2334.3161X＋25.2431（r=0.9999）. The average recovery is 98.6%（RSD=1.36%, n=9） and RSD of reproduction 0.78%. The linear range of glycyrrhizic acid is 0.25-7.50 μg and regression equation is Y=907.7837X＋0.8969（r=0.9999）. The average recovery is 100.2%（RSD=l.48%, n=9） and RSD of reproduction 1.25%. Conclusion： The method is convenient and accurate. It can be used to control the dose limit of glycyrrhiza in similar food.