无梗五加果金丝桃苷的分离制备和鉴定

Isolation,Preparation and Identification of Hyperin from Acanthopanax Sessiliflorus (Rupr.et Maxim.) Seem fruit

采用薄层层析法对树脂纯化后的无梗五加果总黄酮组成成分进行了分析,结果表明无梗五加果中含有药用成分金丝桃苷。应用制备型高效液相色谱和聚酰胺对其进行了制备,采用紫外光谱、红外光谱和核磁共振对高效液相制备的金丝桃苷进行了鉴定。为无梗五加果的开发利用提供了理论依据。

Thin-layer chromatographic procedure was used to identify the composition of flavonoids of Acanthopanax Sessiliflorus （Rupr.et Maxim.） Seem purified by macroporous absorbing resins. The result showed that hyperin is one composition of the flavonoids. The preparative method of hyperin with HPLC and polyamide column were studied and the preparative hyperin was identified by UV, IR and NMR. The results showed that： the purity of the preparative hyperin can be up to 98%. UV, IR and NMR analysis proved that the furit hyperin is exploitable for further develonment.