高效液相色谱法测定马铃薯块茎的绿原酸含量

Determination of Chlorogenic Acid of Potato Tuber by HPLC

目的:建立测定马铃薯块茎中绿原酸含量的高效液相色谱方法,并测定不同品质特点的马铃薯的绿原酸含量。方法:对甲醇浓度、固液比和超声提取时间三个试验因素进行正交试验设计:ZOBAX C_(18)色谱柱,250mm×4．6mm 5μm;流动相:水-甲醇-冰醋酸(20∶80∶2;30∶70∶2;40∶60∶2);甲醇-0．4%磷酸溶液(10∶90):流速: 1．0ml/min;柱温:室温;进样体积:5μl;检测波长为327nm。结果:精密度:RSD为3．2%,n=6,加样回收率为97．5%,提取条件为:甲醇浓度70%,固液比1∶100,超声提取时间40min。确定水∶甲醇∶冰醋酸=30∶70∶2为流动相。

Object： To establish a new HPLC method for determination of chlorogenic acid （CGA） in potato, and determine its content of CGA in five cultivars. Method： Orthogonal experiment for three factors： CH3OH concentration, ratio of solid and liquid and time of ultrasounic extraction. CGA was analyzed with C18 column （250 mm×4.6mm, 5μm） and with detector at 327 nm. The mobile phase consisting H20- CH3OH- CH3COOH（20：80：2; 30：70：2; 40：60：2 respectively）, CH3OH-0.4% I-IaPO4 （10：90）. Results： The recovery of CGA is 97.5%, precision （RSD） 3.2% （n---6）; CGA extraction conditions are： 70% CH3OH, ratio of solid and liquid=l： 100, and ultrasounic extraction time 40 min with determined mobile phase H20-CH3OH- CH3COOH=30： 70：2.