卵巢黏液性肿瘤caspase-3、caspase-9和Smac的表达及其意义

Expression of apoptosis-regulatory proteins caspase-3,caspase-9 and Smac in ovarian mucinous neoplasms

目的探讨凋亡调控因子caspase-3、caspase-9和Smac在卵巢黏液性肿瘤中发生、发展过程中的作用。方法采用免疫组化(SP法)和图像分析技术检测41例卵巢黏液性囊腺瘤、10例卵巢交界性黏液性囊腺瘤、14例卵巢黏液性囊腺癌和24例正常卵巢组织中Smac、caspase-9和caspase-3的表达水平。结果caspase-3、caspase-9和Smac在41例黏液性囊腺瘤中的表达率分别为93%、93%和73.3%;在10例交界性黏液性囊腺瘤中的表达率分别为70%、80%和80%;在14例黏液性囊腺癌中的表达率分别为96%、96%和84%。阳性信号主要定位于细胞质,呈弥漫分布。而在正常卵巢中24例中的表达率分别为83.3%、80.9%和58.3%。caspase-3、caspase-9和Smac在黏液性卵巢肿瘤中的表达增加(P<0.05)。结论caspase-3、caspase-9和Smac可能会成为卵巢黏液性肿瘤治疗的新靶点。

Purpose To investigate the role of apoptosis regulatory proteins caspase-3, caspase-9 and Smac in ovarian mucinous neoplasms. Methods Immunohistochemical technique （SP method ） and image analysis were used to assess the expression levels of caspase-3, caspase-9 and Smac in 41 cases of mucinous cystadenoma, 10 borderline mucinous eystadenoma, 14 mucinous cystoadenocarcinoma and 24 normal ovary. Results The expression rates of apoptosis regulatory proteins caspase-3, caspase-9 and Smac in 41 ovarian mucinous cystadenoma was 93% , 93% and 73.3% , in 10 borderline mucinous cystadenoma, 70% , 80% and 80% , in 14 mutinous cystoadenocarcinoma, 96% , 96% and 84% and in 24 normal ovary, 83.3% , 80.9% and 58.3% , respectively. Expression levels of the apoptosis-regulatory proteins in mucinous ovarian neoplasms were higher than that in normal ovary （ P 〈 0.05 ） . Conclusion Expression levels of the apoptosis-regulatory proteins are increased in ovarian mucinous neoplasms, suggesting that caspase-3, caspase-9 and Smac may become new targets of ovarian mucinous neoplasms treatment.