高效液相色谱法荧光检测红车轴草提取物中的苯并(α)芘

Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector

目的：建立高效液相色谱法荧光检测红车轴草提取物中苯并（α）芘的测定方法。方法：以乙腈-水（70∶30）为流动相,流速为1.0 mL.min^-1,Hypersil C18色谱柱,荧光检测器检测,激发波长（Ex）为368 nm,发射波长（Em）为405 nm。结果：在0.93-37.2 ng.mL-1内峰面积和浓度呈良好线性关系,样品平均回收率为85.2%（n=9）。结论：本法可以快速、准确地测定红车轴草提取物中的苯并（α）芘。

Objective： To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract. Method： Analysis was performed on Hypersil C18 column （4. 6 mm × 200mm, 5μm） with acetonitrile and water （70： 30） as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL · min^-1. Result： The linear range was 0. 93-37.2 mL·min^-1, and r = 0. 999 2 （ n = 5 ）. The average recovery of benzopyrene was 85.2% （n =9）.Conclusion： The method was convenient and accurate with good recovery.