特应性皮炎患者RANTES、MIP-1α及趋化因子受体CCR5测定及其意义

Detection of regulated on activation normal T cell expressed and secreted,macrophage inflammatory protein-1 alpha and chemokine receptor CCR5 in patients with atopic dermatitis

目的探讨调节正常T细胞表达和分泌活性因子(Regulated on activation normal T cell expressed and secre-ted,RANTES),巨噬细胞炎性蛋白(Macrophage inflammatory protein-1,MIP-1α)及趋化因子受体CCR5在特应性皮炎(AD)发病中的作用。方法采集30例AD患者及10例健康对照者血液,分离血清和外周血单个核细胞(PBMC),双抗体夹心ELISA法检测PBMC产生的RANTES、MIP-1α含量,荧光定量PCR检测外周血PBMC表达的CCR5 mRNA水平。结果特应性皮炎患者与健康对照者血清RANTES含量分别为(55.7±3.4)μg/L和(35.6±1.8)μg/L,差异有显著性(t=3.036,P<0.01),且RANTES水平与SCORAD呈正相关(r=0.889,P<0.05);MIP-1α含量分别为(51.8±3.6)μg/L和(44.7±4.3)μg/L,差异有显著性(t=2.465,P<0.05)。CCR5 AD患者为1.284±0.088,健康对照为1.133±0.075,差异有显著性(t=2.752,P<0.05)。结论RANTES和MIP-1α及特异性受体CCR5在特应性皮炎患者中均显著增高,差异有显著性,在AD的发病中可能起重要作用。

Objective To explore the roles of regulated on activation normal T cell expressed and secreted （RANTES）, macrophage inflammatory protein-1 alpha（MIP-1α）and chemokine receptor CCR5 in the pathogenesis of atopic dermatitis. Methods 30 atopic dermatitis and 10 normal controls were collected. Pt3MC were separated from the peripheral blood specimen. ELISA method was used to determine the concentration of RANTES and MIP-1α levels in serum. QRT-PCR was used to detect the expression of CCR5 mRNA in PBMC. Results RANTES and MIP-1α were both higher in patients than that of normal controls （P〈0.01 ,P〈0.05）. Furthermore, there was a significant positive correlation between disease activity and RANTES levels（r=0.889, P〈0.05）. Level of CCR5 mR NA expression was significantly increased in patients than in controls（P〈0.05）. Conclusion Our findings indicate that RANTES, MIP-1α and CCR5 may be involved in the pathogenesis of AD, and the expression level may be a good indicator for the disease activity of AD.