太原地区不同临床型胃疾患与幽门螺杆菌感染及其毒力相关基因cagA关系的探讨

Study on relationship between infection of Helicobacter pylori,cagA^+-Hp and gastric diseases

目的探讨幽门螺杆菌(Hp)感染、cagA+-Hp与胃部疾病严重程度的关系。方法胃镜直视下取102例胃粘膜组织并采血,分离血清。应用Hp-ureA-PCR,ELISA,细菌培养3种方法确定Hp感染例数,用PCR检测cagA-Hp基因。结果102例胃疾患病人,确定Hp感染76例,其中57例检测到cagA-Hp基因,检出率为75.00%。各型胃疾患病人cagA检出率分别为:CSG 52.38%(11/21),CAG 92.31%(12/13),GU81.82%(18/22),GC 80.00%(16/20)。结论1)各种胃部疾病均与Hp感染有关,但单纯的Hp感染不足以解释临床结局的多样性;2)cagA+-Hp与胃部疾患的严重程度相关,携带有cagA的Hp可能具有较强的致病性,但其具体的致病机理尚需进一步阐明。

Objective To investigate the relation between Helicobacter pylori（Hp） infection, cagA positive rate and the severe level of the gastric diseases. Methods Gastric mucosal biopsies of 102 patients were taken by endoscopy and serum of them was collected too. Hp was detected by Hp-ureA-PCR, ELISA and Hp cultivation. Results In the 102 patients, the number of Hp infection was 76, in which 57 were found cagA gene-positive. The detection rate was 75.00 %. The cagA detection rate of patients in each gastric disease were： CSG 52.38% （11/21）, CAG 92.31% （12/13）, GU 81.82% （18/22）, GC 80. 00% （16/20）. Conclusion 1）Gastric disease is associated with Hp infection, but only Hp infection can not explain the variety of clinical outcome thoroughly; 2）There is a close relation between cagA and the degree of gastric disease severity. Hp with cagA may have strong pathogenicity. But the mechanism is not clear.