小鹅瘟的研究——弱毒在雏鹅和成年鹅体内分布和排泄

STUDY ON GOSLING PLAGUE (GP) ——DISTRIBUTION AND EXCREMENT OF ATTENUATED GOSLING PLAGUE VIRUS IN GOOSE

本文首次报道了将小鹅瘟病毒（ＧＰＶ）弱毒株注射鹅（成年鹅和雏鹅）后，用Ｄｏｔ－ＥＬＩＳＡ检测了不同时间病毒在鹅体各组织器官的分布和由粪便排出的情况，以及同群饲养但不注射ＧＰＶ的雏鹅和成年鹅感染ＧＰＶ弱毒的情况。实验结果表明，ＧＰＶ弱毒经肌肉注射到１日龄雏鹅体内后８小时即可在心血中检测到ＧＰＶ的存在，ＧＰＶ弱毒经肌肉注射到成年鹅体内后８小时即可在心血中检测ＧＰＶ的存在；弱毒注射到雏鹅体内后用ＤｏｔＥＬＩＳＡ检测到ＧＰＶ的时间顺序是：心、肝、脾、肺、肾，而胸肌和脑未检测到ＧＰＶ弱毒的存在，而同居感染雏鹅各个组织、器官未检测ＧＰＶ的存在；弱毒注射到成年鹅体内后检测到ＧＰＶ的时间顺序均为：心、肝、肾、脾、肺，同样胸肌和脑均未检测到ＧＰＶ的存在，而同居成年鹅的各个组织、器官未检测到ＧＰＶ的存在。为临床上更好地应用ＧＰ弱毒疫苗预防ＧＰ提供了理论依据。

After attenuated Gosling Plague virus (GPV) was injected into old and young Goose, its distribution and excrement was detected by using DotELISA. And it was detected to the infected old and young Goose that were raised in same flock but no injected (RSF Goose). The test results indicated that: The time sequences that GPV existing in organizes of young duck after injection is heart, liver, spleen, lung, kidney, on GPV was detected in the excrement. There wasn't GPV existing in chest muscle and brain. And there wasn's GPV in chest muscle and brain.The time sequences that GPV existing in organizes of old duck after injection is heart, liver, kidney, spleen, lung. There wasn't GPV existing in chest nuscle and brain, no GPV was detected in the excrement as well. This provided theory basis for attenuated DHV vaccine predicting DHV in clinical.