p16和p53基因在乳腺癌和卵巢癌中表达水平的比较研究

Study of the expression of p16 and p53 gene in breast carcinoma and ovarian carcinoma

目的研究乳腺癌和卵巢癌中p53和p16蛋白的表达,探讨它们之间的关系及其临床意义。方法应用免疫组织化学S-P法,检测38例乳腺癌和39例卵巢癌中p16、p53蛋白的表达。结果乳腺癌中p53和p16基因蛋白异常表达率分别为60．5%、63．2%,卵巢癌中两蛋白异常表达率分别为66．7%、64．1%;差异均无统计学意义（P＞0．05）。p53蛋白阳性率与组织学分级有相关性,随着组织学分级的增加而逐渐增高;p16的变化规律与p53蛋白阳性率的变化恰好相反。p53蛋白与p16蛋白表达率呈显著负相关（r= 79．68．P＜0．05）。结论p16蛋白的缺失表达及p53蛋白的高表达与乳腺癌的发生发展有关,可作为判断乳腺癌生物学行为和预后的重要指标。

Objective To study the expression of p16 and p53 protein and their relationship and clinical significance in breast carcinoma and ovarian carcinoma. Methods The experssion of p16 and p53 protein were detected in 38 cases of breast carcinoma and 39 cases of ovarian arcinom a with immunohistochemical S-P technique. Results The expressions of p53 and p16 proteins were 60.5 % ,63.2 % in breast carcinoma and 66.7 % , 64.1% in the ovarin carcinoma. It had no significance difference（ P 〉 0.05）. The expression rate of p53V ＋ cells of ,he patients increased with the increase-ment of pathology grades. While the expression regularity of p16 ＋ cells in tumor tissues was contrary compared with＇that of p53V. The correlation analysis showed that the expression rate of p53V ＋ cells was negatively correlated to that of p16 ＋ cells （ r - 79.68, P 〈 0.05 ）. Conclusion The loss of p16 protein and over-expression of p53 protein are related to the genesis and development of breast barcinoma and ovarian carcinoma, p16 and p53 protein may be referred to indication to determine biological behavior and prognisis of breast carcinoma and ovarian carcinoma.