反相高效液相色谱法测定三茵舒肝颗粒中绿原酸含量

Determination of chlorogenic acid in Sanyin Shugan granules by RP-HPLC

目的研究建立三茵舒肝颗粒中绿原酸的反相高效液相色谱（RP-HPLC）测定方法。方法采用Diamonsil^TMC18色谱柱（250 mm×4．6 mm,5μm）,流动相为甲醇-0．02%磷酸（30：70）,流速1．0 ml/min,检测波长为327 nm,柱温：30℃。结果绿原酸在16．85～337 ng线性良好（r=0．9993）,加样平均回收率为97．25%（RSD=3．11%）。结论本方法操作简便,结果准确,可用于三茵舒肝颗粒的质量控制。

Objective To establish a method for the determination of chlorogenic acid in Sanyin Shugan Granules by RP-HPLC. Methods Chromatography was performed on a diamonsilTM C18 column （250mm × 4.6mm, Stem）, at 30℃ ; The mobile phase was methaol-0.02 % H3 PO4 （ 30 ： 70 ） ; The flow rate was 1.0ml/min; The detection wavelength was at 327nm. Results The linear range of chlorogenic acid was 16. 85- 337ng （ r = 0. 9993 ）, the average recovery was 97.25 % （RSD = 3.11% ）. Conclusion This method is simple, accurate, feasible and reproducible. It can be used for the quality control of Sanyin Shugan granules.