大岩桐快繁体系优化研究

Study on Optimized System for Rapid Propagation in vitro of Sinningia speciosa

以大岩桐种子无菌萌发获得无菌苗为试材,进行大岩桐快繁体系建立的研究.结果表明:MS+BA 2.0 mg/L是诱导愈伤组织和丛芽形成的合适培养基;MS+BA 1.0 mg/L+NAA 0.2 mg/L是继代增殖的最佳培养基,增殖系数达4.7;诱导生根培养基1/2MS+IBA 0.4 mg/L,生根率达100%,且根系健壮;在腐质土∶椰糠∶珍珠岩=1∶1∶2的混合基质上,生根苗有98%的成活率,移栽苗的生长状况最好,是大岩桐试管苗移栽的合适基质.

This paper studied the optimized media of callus-induction and differentiation, multiplication and root-induction of Sinningia speciosa, using the materials of stems and laminae cut from the plants originated from aseptically germinated seeds. The transplanting techniques were also studied through transplanting the plantlets with healthy roots in six substrates mixed with leaf mold, coconut pollard and perlite. The results showed that the suitable medium to induce calli was MS＋BA2.0 mg/L, and the multiplication medium with the highest multiplication quotient （4. 7） was MS＋BA1.0 mg/L＋NAA0. 2 mg/L. A rooting rate of 100% was achieved on the rooting medium, 1/2 MS added with 0.4 mg/L IBA. The best substrate with a survival rate of 98% was a mixture of leaf mold, coconut pollard and perlite, with a ratio at 1 ：1：2.