中国沙棘和俄罗斯沙棘的ISSR分析

ISSR Analysis of Chinese Seabuckthorn and Russian Seabuckthorn

采用ISSR标记,对中国沙棘和俄罗斯沙棘进行遗传多样性分析,结果显示:(1)13个随机引物共检测到96个位点,其中95个位点具有多态性.(2)中国沙棘雌、雄株的多态位点分别为57和56,多态位点百分率为59.38%和58.33%;俄罗斯沙棘雌、雄株的多态位点均为72,多态位点百分率为75%.(3)Nei指数和Shannon指数估计的遗传多样性表现为俄罗斯沙棘雄株最高,其次为俄罗斯沙棘雌株,然后是中国沙棘雌株,最小的是中国沙棘雄株.表明俄罗斯沙棘因其为蒙古沙棘和中亚沙棘的杂交后代,在遗传上更具有多样性,而中国沙棘由于种群单一,其遗传多样性低;从种间遗传关系来看,中国沙棘的雌株与雄株及俄罗斯沙棘的雌株与雄株的遗传距离近,而中国沙棘与俄罗斯沙棘的遗传距离较远.

The genetic diversity of Chinese seabuckthorn and Russian seabuckthorn was analyzed by ISSR. 96 loci were detected with 13 primers and 95 loci of them were polymorphic. On the genetic polymorphism of individuals in the populations,the female and male of Russian seabuckthorn had the highest polymorphic loci percentage （75%）, then the female of Chinese seabuckthorn （59.83%） and the male of Chinese seabuckthorn （58.33%）. The genetic polymorphism from the Nei and Shannon showed that the male of Russian seabuckthorn was the highest, then the female of Russian seabuckthorn, followed by the female of Chinese seabuckthorn and the male of Chinese seabuckthorn was the lowest. The Russian seabuckthorn is the hybrid of Mongolian seabuckthorn and Middle Asia seabuckthorn, so it had high polymorphism, the Chinese seabuckthorn had low polymorphic because of its simple population. Genetic distance between the female and male of Chinese seabuckthorn was nearer,the female and male of Russian seabuckthorn was also nearer. Genetic distance between the native species and external species was longer.