利用高静水压技术提高黏红酵母生物合成β-胡萝卜素的产量

Increasing production of β-carotene from rhodotorula glutinis by high hydrostatic pressure

利用100-500MPa的高静水压和10-30min的保压时间对黏红酵母（Rh.glutinisRG5）进行处理,发现处理10min时的高压存活曲线呈现马鞍形,说明高静水压处理的最终效应可能是多种生物学效应的累积。在250MPa处理10min所获得的高压突变株RG5-3,其生物量虽然比出发菌株RG5下降了9.25%,但目的产物β-胡萝卜素的含量却比RG5提高了68.60%。传代实验表明该诱变株遗传稳定性良好,没有发生性状退化及回复突变。利用限制性片段长度多态性分析（RFLP）比较发现,限制性内切酶HindⅢ和BamHⅠ切割的RG5和RG5-3的DNA片段有明显不同,初步证明高静水压使黏红酵母RG5在表面性状和DNA水平上都发生了改变。

Rh. glutinis RG5 high-producing t-carotene was treated by ultra high pressure （UHP） of 100- 500 MPa for 10-30 min. The shape of the survival curve was saddle after 10 minutes of treatment, which shows that the result of UHP treatment maybe a cumulation of many biological effects.The biomass of mutant RGS-3（obtained by 250 MPa for 10 rain） was decreased by 9.25% related to the untreated RG5,but its β-carotene content was increased by 68.60% compared to RGS,and its genetic quality was proved to be stable by experiments. The DNA fragments cut by HindⅢ and BamHⅠ were different, and this result proved that UHP made Rh. glutinis RG5 both changed in exterior characters and in nucleic acid level.